• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 85018-4-RR

    WB analysis using 85018-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85018-4-RR (SMARCA2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 85018-4-RR

    IHC staining of human placenta using 85018-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85018-4-RR (SMARCA2-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SMARCA2 antibody included
  • IHC staining of mouse brain using 85018-4-RR

    IHC staining of mouse brain using 85018-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85018-4-RR (SMARCA2-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SMARCA2 antibody included
  • IHC staining of mouse brain using 85018-4-RR

    IHC staining of mouse brain using 85018-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85018-4-RR (SMARCA2-Specific antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SMARCA2 antibody included
  • IF Staining of HeLa using 85018-4-RR

    IF Staining of HeLa using 85018-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMARCA2-Specific antibody (85018-4-RR, Clone: 242511E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85018-4-RR

    Affinity and Kinetic Characterization of 85018-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85018-4-RR against Human SMARCA2-Specific were performed. The affinity constant is 3.86 nM.

SMARCA2-Specific Recombinant monoclonal antibody

SMARCA2-Specific Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85018-4-RR

产品说明书

CloneNo. 242511E1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

SMARCA2, 242511E1, ATP-dependent helicase SMARCA2, BAF190, BAF190B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, U-251 cells, MCF-7 cells, K-562 cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SMARCA2 Ready-To-Use IHC Kit. SMARCA2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85018-4-RR targets SMARCA2-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag25157

Product name: Recombinant human SMARCA2-Specific protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-272 aa of NM_003070

Sequence: MSTPTDPGAMPHPGPSPGPGPSPGPILGPSPGPGPSPGSVHSMMGPSPGPPSVSHPMPTMGSTDFPQEGMHQMHKPIDGIHDKGIVEDIHCGSMKGTGMRPPHPGMGPPQSPMDQHSQGYMSPHPSPLGAPEHVSSPMSGGGPTPPQMPPSQPGALIPGDPQAMSQPNRGPSPFSPVQLHQLRAQILAYKMLARGQPLPETLQLAVQGKRTLPGLQQQQQQQQQQQQQQQQQQQQQQQPQQQPPQPQTQQQQQPALVNYNRPSGPGPELSGP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
别名 SMARCA2, 242511E1, ATP-dependent helicase SMARCA2, BAF190, BAF190B
计算分子量 181 kDa
观测分子量210 kDa
GenBank蛋白编号NM_003070
基因名称 SMARCA2
Gene ID (NCBI) 6595
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP51531
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMARCA2 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

实验方案

Product Specific Protocols
IF protocol for SMARCA2-Specific antibody 85018-4-RRDownload protocol
WB protocol for SMARCA2-Specific antibody 85018-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...