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验证数据展示

  • WB analysis using 85018-4-RR

    WB analysis using 85018-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85018-4-RR (SMARCA2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 85018-4-RR

    IF Staining of HeLa using 85018-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMARCA2-Specific antibody (85018-4-RR, Clone: 242511E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85018-4-RR

    Affinity and Kinetic Characterization of 85018-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85018-4-RR against Human SMARCA2-Specific were performed. The affinity constant is 3.86 nM.

SMARCA2-Specific Recombinant antibody

SMARCA2-Specific Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85018-4-RR

产品说明书

CloneNo. 242511E1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

SMARCA2, BRM, BRG1-associated factor 190B, BAF190B, BAF190

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, U-251 cells, MCF-7 cells, K-562 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SMARCA2 Ready-To-Use IHC Kit. SMARCA2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85018-4-RR targets SMARCA2-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SMARCA2-Specific fusion protein Ag25157 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
别名 SMARCA2, BRM, BRG1-associated factor 190B, BAF190B, BAF190
计算分子量 181 kDa
观测分子量210 kDa
GenBank蛋白编号NM_003070
基因名称 SMARCA2
Gene ID (NCBI) 6595
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP51531
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMARCA2 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

实验方案

Product Specific Protocols
WB protocol for SMARCA2-Specific antibody 85018-4-RRDownload protocol
IF protocol for SMARCA2-Specific antibody 85018-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols