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验证数据展示

  • IF Staining of U2OS using CL488-83225-2

    IF Staining of U2OS using CL488-83225-2

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 SLC12A8 antibody (CL488-83225-2, Clone: 240082B11 ) at dilution of 1:200.

  • FC experiment of U-251 using CL488-83225-2

    FC experiment of U-251 using CL488-83225-2

    1x10^6 U-251 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated SLC12A8 Recombinant antibody (CL488-83225-2, Clone:240082B11)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated SLC12A8 Recombinant monoclonal antibody

SLC12A8 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-83225-2

产品说明书

CloneNo. 240082B11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

CCC9, Cation-chloride cotransporter 9, 240082B11

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU-251 cells
For other applications, we recommend the unconjugated version of this antibody, 83225-2-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83225-2 targets SLC12A8 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag34354

Product name: Recombinant human SLC12A8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-186 aa of BC020506

Sequence: MCSCSLTPVPEPVLREGAEGLHCSEHLLLEKAPSYGSEGPAQRVLEGTLLEFTKDMDQLLQLTRKLESSQPRQGEGNRTPESQKRKSKKATKQTLQDSFLLDLKSPPSFPVEISDRLPAASWEGQESCWNKQTSKSEGTQPEGTYGEQLVPELCNQSESSGEDFFLKSRLQEQDVWRRSTSFYTHM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 12 (potassium/chloride transporters), member 8
别名 CCC9, Cation-chloride cotransporter 9, 240082B11
计算分子量 714 aa, 80 kDa
GenBank蛋白编号BC020506
基因名称 SLC12A8
Gene ID (NCBI) 84561
RRIDAB_3673223
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDA0AV02
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SLC12A8 antibody CL488-83225-2Download protocol
IF protocol for CL Plus 488 SLC12A8 antibody CL488-83225-2Download protocol
Standard Protocols
Click here to view our Standard Protocols
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