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验证数据展示

  • IF Staining of U2OS using 83225-2-RR

    IF Staining of U2OS using 83225-2-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SLC12A8 antibody (83225-2-RR, Clone: 240082B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of human intrahepatic cholangiocarcinoma using 83225-2-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 83225-2-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83225-2-RR (SLC12A8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of MCF-7 using 83225-2-RR

    FC experiment of MCF-7 using 83225-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug SLC12A8 Recombinant antibody (83225-2-RR, Clone:240082B11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83225-2-RR

    Affinity and Kinetic Characterization of 83225-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83225-2-RR against Human SLC12A8 were performed. The affinity constant is 3.03 nM.

SLC12A8 Recombinant monoclonal antibody

SLC12A8 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83225-2-RR

产品说明书

CloneNo. 240082B11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

CCC9, Cation-chloride cotransporter 9, 240082B11

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83225-2-RR targets SLC12A8 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34354

Product name: Recombinant human SLC12A8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-186 aa of BC020506

Sequence: MCSCSLTPVPEPVLREGAEGLHCSEHLLLEKAPSYGSEGPAQRVLEGTLLEFTKDMDQLLQLTRKLESSQPRQGEGNRTPESQKRKSKKATKQTLQDSFLLDLKSPPSFPVEISDRLPAASWEGQESCWNKQTSKSEGTQPEGTYGEQLVPELCNQSESSGEDFFLKSRLQEQDVWRRSTSFYTHM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 12 (potassium/chloride transporters), member 8
别名 CCC9, Cation-chloride cotransporter 9, 240082B11
计算分子量 714 aa, 80 kDa
GenBank蛋白编号BC020506
基因名称 SLC12A8
Gene ID (NCBI) 84561
RRIDAB_3670909
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDA0AV02
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for SLC12A8 antibody 83225-2-RRDownload protocol
IHC protocol for SLC12A8 antibody 83225-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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