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验证数据展示

  • WB analysis of HeLa using 15641-1-AP (same clone as 15641-1-PBS)

    WB analysis of HeLa using 15641-1-AP (same clone as 15641-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15641-1-AP (SH2D3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15641-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 15641-1-AP (same clone as 15641-1-PBS)

    IHC staining of human ovary cancer using 15641-1-AP (same clone as 15641-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15641-1-AP (SH2D3A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15641-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 15641-1-AP (same clone as 15641-1-PBS)

    IF Staining of HeLa using 15641-1-AP (same clone as 15641-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SH2D3A antibody (15641-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 15641-1-PBS in a different storage buffer formulation.

SH2D3A Polyclonal antibody, PBS Only

SH2D3A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15641-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

UNQ175/PRO201, SH2 domain-containing protein 3A, NSP1, Novel SH2-containing protein 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15641-1-PBS targets SH2D3A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8132

Product name: Recombinant human NSP1;SH2D3A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC006281

Sequence: MQVPQDGEDLAGQPWYHGLLSRQKAEALLQQNGDFLVRASGSRGGNPVISCRWRGSALHFEVFRVALRPRPGRPTALFQLEDEQFPSIPALVHSYMTGRRPLSQATGAVVSRPVTWQGPLRRSFSEDTLMDGPARIEPLRARKWSNSQPADLAHMGRSREDPAGMEASTMPISALPRTSSDPVLLKAPAPLGTVADSLRASDGQLQAKAPTKPPRTPSFELPDASERPPTYCELVPRVPSVQGTSPSQSCPEPEAPWWEAEEDEEEENRCFTRPQAEISFCPHDAPSCLLGPQNRPLEPQVLHTLRGLFLEHHPGSTALHLLLVDCQATGLLGVTRDQRGNMGVSSGLEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH2 domain containing 3A
别名 UNQ175/PRO201, SH2 domain-containing protein 3A, NSP1, Novel SH2-containing protein 1
计算分子量 576 aa, 63 kDa
观测分子量 64 kDa
GenBank蛋白编号BC006281
基因名称 SH2D3A
Gene ID (NCBI) 10045
RRIDAB_2286019
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BRG2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SH2 domain containing 3A (SH2D3A) Is is a novel SH2-containing protein 1 (NSP1) with proline-rich domains and therefore probably involved in the signal-transduction of some ligand-activated tyrosine kinase receptors. Three phosphorylation sites of NSP1 have been found to date. NSP1 may be located at cell membrane play a role in JNK activation.

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