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SH2D3A Polyclonal antibody

SH2D3A Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 15641-1-AP

Print datasheet

Synonyms

Novel SH2 containing protein 1, NSP1, SH2 domain containing 3A, SH2D3A

验证数据展示

  • WB analysis of HeLa using 15641-1-AP

    WB analysis of HeLa using 15641-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15641-1-AP (SH2D3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15641-1-AP

    IF Staining of HeLa using 15641-1-AP

    Immunofluorescent analysis of HeLa cells using 15641-1-AP (SH2D3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15641-1-AP targets SH2D3A in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB,ELISA
Tested Reactivity human
Immunogen SH2D3A fusion protein Ag8132 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SH2 domain containing 3A
Synonyms Novel SH2 containing protein 1, NSP1, SH2 domain containing 3A, SH2D3A
Calculated Molecular Weight 576 aa, 63 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC006281
Gene Symbol SH2D3A
Gene ID (NCBI) 10045
RRIDAB_2286019
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRG2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SH2 domain containing 3A (SH2D3A) Is is a novel SH2-containing protein 1 (NSP1) with proline-rich domains and therefore probably involved in the signal-transduction of some ligand-activated tyrosine kinase receptors. Three phosphorylation sites of NSP1 have been found to date. NSP1 may be located at cell membrane play a role in JNK activation.

实验方案

Product Specific Protocols
WB protocol for SH2D3A antibody 15641-1-APDownload protocol
IF protocol for SH2D3A antibody 15641-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols