验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
15641-1-AP targets SH2D3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag8132 Product name: Recombinant human NSP1;SH2D3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC006281 Sequence: MQVPQDGEDLAGQPWYHGLLSRQKAEALLQQNGDFLVRASGSRGGNPVISCRWRGSALHFEVFRVALRPRPGRPTALFQLEDEQFPSIPALVHSYMTGRRPLSQATGAVVSRPVTWQGPLRRSFSEDTLMDGPARIEPLRARKWSNSQPADLAHMGRSREDPAGMEASTMPISALPRTSSDPVLLKAPAPLGTVADSLRASDGQLQAKAPTKPPRTPSFELPDASERPPTYCELVPRVPSVQGTSPSQSCPEPEAPWWEAEEDEEEENRCFTRPQAEISFCPHDAPSCLLGPQNRPLEPQVLHTLRGLFLEHHPGSTALHLLLVDCQATGLLGVTRDQRGNMGVSSGLEL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SH2 domain containing 3A |
| 别名 | UNQ175/PRO201, SH2 domain-containing protein 3A, NSP1, Novel SH2-containing protein 1 |
| 计算分子量 | 576 aa, 63 kDa |
| 观测分子量 | 64 kDa |
| GenBank蛋白编号 | BC006281 |
| 基因名称 | SH2D3A |
| Gene ID (NCBI) | 10045 |
| RRID | AB_2286019 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BRG2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SH2 domain containing 3A (SH2D3A) Is is a novel SH2-containing protein 1 (NSP1) with proline-rich domains and therefore probably involved in the signal-transduction of some ligand-activated tyrosine kinase receptors. Three phosphorylation sites of NSP1 have been found to date. NSP1 may be located at cell membrane play a role in JNK activation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SH2D3A antibody 15641-1-AP | Download protocol |
| IHC protocol for SH2D3A antibody 15641-1-AP | Download protocol |
| WB protocol for SH2D3A antibody 15641-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
