验证数据展示
经过测试的应用
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66328 targets SFRP2 in IF/ICC applications and shows reactivity with human, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag18840 Product name: Recombinant human SFRP2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-295 aa of BC008666 Sequence: MLQGPGSLLLLFLASHCCLGSARGLFLFGQPDFSYKRSNCKPIPVNLQLCHGIEYQNMRLPNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSLCVQVKDRCAPVMSAFGFPWPDMLECDRFPQDNDLCIPLASSDHLLPATEEAPKVCEACKNKNDDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERDLKKSVLWLKDSLQCTCEEMNDINAPYLVMGQKQGGELVITSVKRWQKGQREFKRISRSIRKLQC 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | secreted frizzled-related protein 2 |
| 别名 | FRP 2, FRP2, SARP 1, SARP1, SDF 5, sFRP 2, SFRP2, UNQ361/PRO697 |
| 计算分子量 | 295 aa, 34 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC008666 |
| 基因名称 | SFRP2 |
| Gene ID (NCBI) | 6423 |
| RRID | AB_2883310 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q96HF1 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SFRP2 antibody CL488-66328 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
