CoraLite® Plus 488-conjugated SFRP2 Monoclonal antibody
SFRP2 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1F3A1
验证数据展示
经过测试的应用
| Positive IF detected in | SH-SY5Y cells |
For other applications, we recommend the unconjugated version of this antibody, 66328-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66328 targets SFRP2 in IF applications and shows reactivity with human, rat samples.
| Tested Applications | IF/ICC |
| Tested Reactivity | human, rat |
| Immunogen | SFRP2 fusion protein Ag18840 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | secreted frizzled-related protein 2 |
| Synonyms | FRP 2, FRP2, SARP 1, SARP1, SDF 5, sFRP 2, SFRP2, UNQ361/PRO697 |
| Calculated Molecular Weight | 295 aa, 34 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | BC008666 |
| Gene Symbol | SFRP2 |
| Gene ID (NCBI) | 6423 |
| RRID | AB_2883310 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q96HF1 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SFRP2 antibody CL488-66328 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |