SFRP2 Monoclonal antibody

SFRP2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66328-1-Ig

产品说明书

CloneNo. 1F3A1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, ELISA

1F3A1, FKSG12, SARP1, SDF-5, Secreted apoptosis-related protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存


经过测试的应用

Positive WB detected inpig brain tissue, Y79 cells, COLO 320 cells, rat brain tissue, rabbit brain tissue, mouse brain tissue
Positive IHC detected inhuman malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66328-1-Ig targets SFRP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat, pig, rabbit
文献引用反应性human
免疫原 SFRP2 fusion protein Ag18840 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 secreted frizzled-related protein 2
别名 1F3A1, FKSG12, SARP1, SDF-5, Secreted apoptosis-related protein 1
计算分子量 295 aa, 34 kDa
观测分子量 38 kDa
GenBank蛋白编号BC008666
基因名称 SFRP2
Gene ID (NCBI) 6423
RRIDAB_2881709
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96HF1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.

实验方案

Product Specific Protocols
WB protocol for SFRP2 antibody 66328-1-IgDownload protocol
IHC protocol for SFRP2 antibody 66328-1-IgDownload protocol
IF protocol for SFRP2 antibody 66328-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Exp Cell Res

miR-629-5p promotes growth and metastasis of hepatocellular carcinoma by activating β-catenin.

Authors - Xin Tao
Human

Front Immunol

Integrated single-cell RNA sequencing reveals the tumor heterogeneity and microenvironment landscape during liver metastasis in adenocarcinoma of esophagogastric junction

Authors - Junrui Xu
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