• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66328-1-Ig (same clone as 66328-1-PBS)

    WB analysis using 66328-1-Ig (same clone as 66328-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51471-1

    Cytometric bead array sample test of MP51471-1

    Cytometric bead array sample test of MP51471-1, SFRP2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66328-2-PBS. Detection antibody: 66328-1-PBS.

  • IHC staining of human malignant melanoma using 66328-1-Ig (same clone as 66328-1-PBS)

    IHC staining of human malignant melanoma using 66328-1-Ig (same clone as 66328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66328-1-Ig (SFRP2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

  • IHC staining of human malignant melanoma using 66328-1-Ig (same clone as 66328-1-PBS)

    IHC staining of human malignant melanoma using 66328-1-Ig (same clone as 66328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66328-1-Ig (SFRP2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 66328-1-Ig (same clone as 66328-1-PBS)

    IF Staining of A549 using 66328-1-Ig (same clone as 66328-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using SFRP2 antibody (66328-1-Ig, Clone: 1F3A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

  • WB analysis of rat brain using 66328-1-Ig (same clone as 66328-1-PBS)

    WB analysis of rat brain using 66328-1-Ig (same clone as 66328-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51471-1

    Cytometric bead array standard curve of MP51471-1

    Cytometric bead array standard curve of MP51471-1, SFRP2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66328-2-PBS. Detection antibody: 66328-1-PBS. Standard:Ag18840. Range: 0.781-100 ng/mL.

SFRP2 Monoclonal antibody, PBS Only (Detector)

SFRP2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66328-1-PBS

产品说明书

CloneNo. 1F3A1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

1F3A1, FKSG12, SARP1, SDF-5, Secreted apoptosis-related protein 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

66328-1-PBS targets SFRP2 as part of a matched antibody pair:

MP51471-1: 66328-2-PBS capture and 66328-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag18840

Product name: Recombinant human SFRP2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-295 aa of BC008666

Sequence: MLQGPGSLLLLFLASHCCLGSARGLFLFGQPDFSYKRSNCKPIPVNLQLCHGIEYQNMRLPNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSLCVQVKDRCAPVMSAFGFPWPDMLECDRFPQDNDLCIPLASSDHLLPATEEAPKVCEACKNKNDDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERDLKKSVLWLKDSLQCTCEEMNDINAPYLVMGQKQGGELVITSVKRWQKGQREFKRISRSIRKLQC

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 secreted frizzled-related protein 2
别名 1F3A1, FKSG12, SARP1, SDF-5, Secreted apoptosis-related protein 1
计算分子量 295 aa, 34 kDa
观测分子量 38 kDa
GenBank蛋白编号BC008666
基因名称 SFRP2
Gene ID (NCBI) 6423
RRIDAB_2881709
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96HF1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.

||
New chat Able
正在加载，请稍候...