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验证数据展示

  • WB analysis using 85037-5-RR

    WB analysis using 85037-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85037-5-RR (SEC22B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of SH-SY5Y using 85037-5-RR

    FC experiment of SH-SY5Y using 85037-5-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SEC22B Recombinant antibody (85037-5-RR, Clone:242659B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 85037-5-RR

    Affinity and Kinetic Characterization of 85037-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85037-5-RR against Human SEC22B were performed. The affinity constant is 2.07 nM.

SEC22B Recombinant antibody

SEC22B Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 85037-5-RR

产品说明书

CloneNo. 242659B3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, hamster

应用

WB, FC (Intra), ELISA

别名

242659B3, ER-Golgi SNARE of 24 kDa, ERS24, ERS-24, SEC22 vesicle-trafficking protein homolog B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, CHO cells, mouse brain tissue, rat brain tissue, fetal human brain tissue, human placenta tissue
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy SEC22B Ready-To-Use IHC Kit. SEC22B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85037-5-RR targets SEC22B in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, hamster
免疫原 SEC22B fusion protein Ag12981 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SEC22 vesicle trafficking protein homolog B (S. cerevisiae)
别名 242659B3, ER-Golgi SNARE of 24 kDa, ERS24, ERS-24, SEC22 vesicle-trafficking protein homolog B
计算分子量 25 kDa
观测分子量 24 kDa
GenBank蛋白编号BC001364
基因名称 SEC22B
Gene ID (NCBI) 9554
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75396
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor (SNARE) proteins are critical components of the membrane fusion machinery (PMID: 20163564). SEC22B is a SNARE involved in endoplasmic reticulum (ER)/Golgi membrane trafficking. SEC22B localizes to the ER-Golgi intermediate compartment (ERGIC) and has been identified as a regulator of antigen crosspresentation (PMID: 22153078).

实验方案

Product Specific Protocols
WB protocol for SEC22B antibody 85037-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols