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验证数据展示

  • WB analysis using 85037-5-RR (same clone as 85037-5-PBS)

    WB analysis using 85037-5-RR (same clone as 85037-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85037-5-RR (SEC22B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85037-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 85037-5-RR (same clone as 85037-5-PBS)

    IHC staining of mouse liver using 85037-5-RR (same clone as 85037-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85037-5-RR (SEC22B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85037-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SEC22B antibody included
  • IHC staining of mouse liver using 85037-5-RR (same clone as 85037-5-PBS)

    IHC staining of mouse liver using 85037-5-RR (same clone as 85037-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85037-5-RR (SEC22B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85037-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SEC22B antibody included
  • FC experiment of SH-SY5Y using 85037-5-RR (same clone as 85037-5-PBS)

    FC experiment of SH-SY5Y using 85037-5-RR (same clone as 85037-5-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SEC22B Recombinant antibody (85037-5-RR, Clone:242659B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 85037-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85037-5-RR

    Affinity and Kinetic Characterization of 85037-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85037-5-RR against Human SEC22B were performed. The affinity constant is 2.07 nM.

SEC22B Recombinant monoclonal antibody, PBS Only

SEC22B Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 85037-5-PBS

产品说明书

CloneNo. 242659B3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, hamster

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

242659B3, ER-Golgi SNARE of 24 kDa, ERS24, ERS-24, SEC22 vesicle-trafficking protein homolog B

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SEC22B Ready-To-Use IHC Kit. SEC22B primary antibody included.

产品信息

85037-5-PBS targets SEC22B in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, hamster
免疫原

CatNo: Ag12981

Product name: Recombinant human SEC22B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-215 aa of BC001364

Sequence: VLLTMIARVADGLPLAASMQEDEQSGRDLQQYQSQAKQLFRKLNEQSPTRCTLEAGAMTFHYIIEQGVCYLVLCEAAFPKKLAFAYLEDLHSEFDEQHGKKVPTVSRPYSFIEFDTFIQKTKKLYIDSRARRNLGSINTELQDVQRIMVANIEEVLQRGEALSALDSKANNLSSLSKKYRQDAKYLNMRSTYAKLAAVAVFFIMLIVYVRFWWL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SEC22 vesicle trafficking protein homolog B (S. cerevisiae)
别名 242659B3, ER-Golgi SNARE of 24 kDa, ERS24, ERS-24, SEC22 vesicle-trafficking protein homolog B
计算分子量 25 kDa
观测分子量 24 kDa
GenBank蛋白编号BC001364
基因名称 SEC22B
Gene ID (NCBI) 9554
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO75396
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor (SNARE) proteins are critical components of the membrane fusion machinery (PMID: 20163564). SEC22B is a SNARE involved in endoplasmic reticulum (ER)/Golgi membrane trafficking. SEC22B localizes to the ER-Golgi intermediate compartment (ERGIC) and has been identified as a regulator of antigen crosspresentation (PMID: 22153078).

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