• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 86196-1-RR

    WB analysis using 86196-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat liver using 86196-1-RR

    WB analysis of rat liver using 86196-1-RR

    rat liver tissue were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 86196-1-RR

    WB analysis of K-562 using 86196-1-RR

    K-562 cells were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat brain using 86196-1-RR

    WB analysis of rat brain using 86196-1-RR

    rat brain tissue were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of U-87 MG using 86196-1-RR

    WB analysis of U-87 MG using 86196-1-RR

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 86196-1-RR

    WB analysis of NIH/3T3 using 86196-1-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 86196-1-RR

    IF Staining of HEK-293 using 86196-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SCPX antibody (86196-1-RR, Clone: 250813B3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86196-1-RR

    Affinity and Kinetic Characterization of 86196-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86196-1-RR against Human SCPX were performed. The affinity constant is below 1 pM.

SCPX Recombinant antibody

SCPX Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86196-1-RR

产品说明书

CloneNo. 250813B3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

SCP2, SCP2/SCPx, Acetyl-CoA C-myristoyltransferase, EC:2.3.1.155, EC:2.3.1.16

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inrat liver tissue, HepG2 cells, K-562 cells, NIH/3T3 cells, rat brain tissue, U-87 MG cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86196-1-RR targets SCPX in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SCPX fusion protein Ag5895 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sterol carrier protein 2
别名 SCP2, SCP2/SCPx, Acetyl-CoA C-myristoyltransferase, EC:2.3.1.155, EC:2.3.1.16
计算分子量 59 kDa
观测分子量46 kDa, 58 kDa
GenBank蛋白编号BC067108
基因名称 SCP2
Gene ID (NCBI) 6342
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP22307
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SCPX protein is a kind of protein related to cholesterol absorption and transport, belonging to SCP-2 gene family. SCPX protein contains an N-terminal thiolase domain and a C-terminal SCP-2 domain. In cells, SCPX protein will be cut into 44-46 kDa thiolase and 13-15 kDa SCP-2 protein. SCPX-2 is a nonspecific lipid transporter, which is involved in the transport and metabolism of intracellular lipids. It is distributed in peroxisome, cytoplasm and nucleus, and may play a role as a transcription factor in nucleus.

实验方案

Product Specific Protocols
WB protocol for SCPX antibody 86196-1-RRDownload protocol
IF protocol for SCPX antibody 86196-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols