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验证数据展示

  • WB analysis using 86196-1-RR (same clone as 86196-1-PBS)

    WB analysis using 86196-1-RR (same clone as 86196-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86196-1-PBS in a different storage buffer formulation.

  • WB analysis of rat liver using 86196-1-RR (same clone as 86196-1-PBS)

    WB analysis of rat liver using 86196-1-RR (same clone as 86196-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86196-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 86196-1-RR (same clone as 86196-1-PBS)

    WB analysis of K-562 using 86196-1-RR (same clone as 86196-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86196-1-PBS in a different storage buffer formulation.

  • WB analysis of rat brain using 86196-1-RR (same clone as 86196-1-PBS)

    WB analysis of rat brain using 86196-1-RR (same clone as 86196-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86196-1-PBS in a different storage buffer formulation.

  • WB analysis of U-87 MG using 86196-1-RR (same clone as 86196-1-PBS)

    WB analysis of U-87 MG using 86196-1-RR (same clone as 86196-1-PBS)

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86196-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 86196-1-RR (same clone as 86196-1-PBS)

    WB analysis of NIH/3T3 using 86196-1-RR (same clone as 86196-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86196-1-RR (SCPX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86196-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP02291-1

    Sandwich ELISA standard curve of MP02291-1

    Sandwich ELISA standard curve of MP02291-1, Human SCP2/SCPx Recombinant Matched Antibody Pair - PBS only. 86196-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag5895. 86196-1-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

  • Sample test of MP02291-1

    Sample test of MP02291-1

    The mean SCP2/SCPx concentration was determined to be 109.35 ng/mL in A549 cell extract based on a 1.20 mg/mL extract load and 125.11 ng/mL in HepG2 cell extract based on a 1.10 mg/mL extract load.

  • Sample test of MP02291-1

    Sample test of MP02291-1

    The mean SCP2/SCPx concentration was determined to be 249.0 ng/mL in HepG2 cell extract based on a 0.9 mg/mL extract load.

  • IF Staining of HEK-293 using 86196-1-RR (same clone as 86196-1-PBS)

    IF Staining of HEK-293 using 86196-1-RR (same clone as 86196-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SCPX antibody (86196-1-RR, Clone: 250813B3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86196-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP02291-1

    Cytometric bead array standard curve of MP02291-1

    Cytometric bead array standard curve of MP02291-1, SCP2/SCPx Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86196-2-PBS. Detection antibody: 86196-1-PBS. Standard: Ag5895. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 86196-1-RR

    Affinity and Kinetic Characterization of 86196-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86196-1-RR against Human SCPX were performed. The affinity constant is below 1 pM.

SCPX Recombinant antibody, PBS Only (Detector)

SCPX Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 86196-1-PBS

产品说明书

CloneNo. 250813B3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

SCP2, SCP2/SCPx, Acetyl-CoA C-myristoyltransferase, EC:2.3.1.155, EC:2.3.1.16

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86196-1-PBS targets SCPX as part of a matched antibody pair:

MP02291-1: 86196-2-PBS capture and 86196-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 SCPX fusion protein Ag5895 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sterol carrier protein 2
别名 SCP2, SCP2/SCPx, Acetyl-CoA C-myristoyltransferase, EC:2.3.1.155, EC:2.3.1.16
计算分子量 59 kDa
观测分子量46 kDa, 58 kDa
GenBank蛋白编号BC067108
基因名称 SCP2
Gene ID (NCBI) 6342
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP22307
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SCPX protein is a kind of protein related to cholesterol absorption and transport, belonging to SCP-2 gene family. SCPX protein contains an N-terminal thiolase domain and a C-terminal SCP-2 domain. In cells, SCPX protein will be cut into 44-46 kDa thiolase and 13-15 kDa SCP-2 protein. SCPX-2 is a nonspecific lipid transporter, which is involved in the transport and metabolism of intracellular lipids. It is distributed in peroxisome, cytoplasm and nucleus, and may play a role as a transcription factor in nucleus.