验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | U2OS cells |
For other applications, we recommend the unconjugated version of this antibody, 82876-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82876 targets SAGE1 in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag34216 Product name: Recombinant human SAGE1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 247-387 aa of NM_018666.2 Sequence: ATITYNVPEEKMEKGQPQPDNILSTASTGLINVAGAGTPAISTNGLYSTVPHNVCEEKMENDQPQPNNVLSTVQPVIIYLTATGIPGMNTRDQYATITHNVCEERVVNNQPLPSNALSTVLPGLAYLATADMPAMSTRDQH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | sarcoma antigen 1 |
| 别名 | sarcoma antigen 1, SAGE, CT14, Cancer/testis antigen 14, 230094C11 |
| 计算分子量 | 99kd |
| GenBank蛋白编号 | NM_018666.2 |
| 基因名称 | SAGE1 |
| Gene ID (NCBI) | 55511 |
| RRID | AB_3673092 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9NXZ1 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sarcoma antigen 1 (SAGE1) is a cancer/testis antigen that is limited to the spermatogonia and pre-meiotic spermatocytes and localized to the nuclei (PMID: 34465596).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 SAGE1 antibody CL488-82876 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
