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验证数据展示

  • IF Staining of MDA-MB-453 using 82876-1-RR (same clone as 82876-1-PBS)

    IF Staining of MDA-MB-453 using 82876-1-RR (same clone as 82876-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MDA-MB-453 cells using SAGE1 antibody (82876-1-RR, Clone: 230094C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82876-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 82876-1-RR (same clone as 82876-1-PBS)

    FC experiment of U2OS using 82876-1-RR (same clone as 82876-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human SAGE1 (82876-1-RR, Clone:230094C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82876-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82876-1-RR

    Affinity and Kinetic Characterization of 82876-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82876-1-RR against Human SAGE1 were performed. The affinity constant is 0.546 nM.

SAGE1 Recombinant monoclonal antibody, PBS Only

SAGE1 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82876-1-PBS

产品说明书

CloneNo. 230094C11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Indirect ELISA

别名

230094C11, Cancer/testis antigen 14, CT14, SAGE, sarcoma antigen 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82876-1-PBS targets SAGE1 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34216

Product name: Recombinant human SAGE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 247-387 aa of NM_018666.2

Sequence: ATITYNVPEEKMEKGQPQPDNILSTASTGLINVAGAGTPAISTNGLYSTVPHNVCEEKMENDQPQPNNVLSTVQPVIIYLTATGIPGMNTRDQYATITHNVCEERVVNNQPLPSNALSTVLPGLAYLATADMPAMSTRDQH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sarcoma antigen 1
别名 230094C11, Cancer/testis antigen 14, CT14, SAGE, sarcoma antigen 1
计算分子量99kd
GenBank蛋白编号NM_018666.2
基因名称 SAGE1
Gene ID (NCBI) 55511
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NXZ1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sarcoma antigen 1 (SAGE1) is a cancer/testis antigen that is limited to the spermatogonia and pre-meiotic spermatocytes and localized to the nuclei (PMID: 34465596).

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