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验证数据展示

  • IF Staining of HepG2 using CL488-66098

    IF Staining of HepG2 using CL488-66098

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 S100A6 antibody (CL488-66098, Clone: 3D11C8 ) at dilution of 1:200.

  • IF Staining of SH-SY5Y using CL488-66098

    IF Staining of SH-SY5Y using CL488-66098

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 S100A6 antibody (CL488-66098, Clone: 3D11C8 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated S100A6 Monoclonal antibody

S100A6 Monoclonal Antibody for IF/ICC
Cat No. CL488-66098

产品说明书

CloneNo. 3D11C8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC

别名

2A9, 5B10, CABP, CACY, Calcyclin, MLN 4, PRA, Protein S100 A6, S100A6

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells, SH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy S100A6 Ready-To-Use IHC Kit. S100A6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66098-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66098 targets S100A6 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Ag17606

Product name: Recombinant human S100A6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-90 aa of BC001431

Sequence: MACPLDQAIGLLVAIFHKYSGREGDKHTLSKKELKELIQKELTIGSKLQDAEIARLMEDLDRNKDQEVNFQEYVTFLGALALIYNEALKG

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 S100 calcium binding protein A6
别名 2A9, 5B10, CABP, CACY, Calcyclin, MLN 4, PRA, Protein S100 A6, S100A6
计算分子量 10 kDa
观测分子量 10 kDa
GenBank蛋白编号BC001431
基因名称 S100A6
Gene ID (NCBI) 6277
RRIDAB_3084204
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP06703
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 S100A6 antibody CL488-66098Download protocol
Standard Protocols
Click here to view our Standard Protocols
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