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S100A6 Monoclonal antibody

S100A6 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3D11C8

Cat No : 66098-1-Ig

Print datasheet

Synonyms

2A9, 5B10, CABP, CACY, Calcyclin, MLN 4, PRA, Protein S100 A6, S100A6

验证数据展示

  • WB analysis of HeLa using 66098-1-Ig

    WB analysis of HeLa using 66098-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66098-1-Ig (S100A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66098-1-Ig

    IF Staining of HepG2 using 66098-1-Ig

    Immunofluorescent analysis of HepG2 cells, using S100A6 antibody 66098-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

  • IHC staining of human colon cancer using 66098-1-Ig

    IHC staining of human colon cancer using 66098-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A6 antibody included
  • IHC staining of human colon cancer using 66098-1-Ig

    IHC staining of human colon cancer using 66098-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A6 antibody included

经过测试的应用

Positive WB detected inHeLa cells, A549 cells
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy S100A6 Ready-To-Use IHC Kit. S100A6 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 66098-1-Ig is S100A6 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen S100A6 fusion protein Ag17606 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name S100 calcium binding protein A6
Synonyms 2A9, 5B10, CABP, CACY, Calcyclin, MLN 4, PRA, Protein S100 A6, S100A6
Calculated Molecular Weight 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC001431
Gene Symbol S100A6
Gene ID (NCBI) 6277
RRIDAB_2881497
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06703
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis.

实验方案

Product Specific Protocols
WB protocol for S100A6 antibody 66098-1-IgDownload protocol
IHC protocol for S100A6 antibody 66098-1-IgDownload protocol
IF protocol for S100A6 antibody 66098-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Open Med (Wars)

S100A6 promotes proliferation and migration of HepG2 cells via increased ubiquitin-dependent degradation of p53.

Authors - Dongqiang Song
View Article