CoraLite®594-conjugated RRM2 Monoclonal antibody

RRM2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67006

产品说明书

CloneNo. 2A9A7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

RR2M, RR2, R2, EC:1.17.4.1, 2A9A7

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RRM2 Ready-To-Use IHC Kit. RRM2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67006-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67006 targets RRM2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 RRM2 fusion protein Ag28664 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ribonucleotide reductase M2 polypeptide
别名 RR2M, RR2, R2, EC:1.17.4.1, 2A9A7
计算分子量 389 aa, 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC030154
基因名称 RRM2
Gene ID (NCBI) 6241
RRIDAB_3084742
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP31350
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribonucleotide reductase M2 subunit is one of two subunits that constitute ribonucleotide reductase, the enzyme that catalyzes the conversion of ribonucleotide 5'-diphosphates into 2'-deoxyribonucleotides, a rate-limiting step in the production of 2'-deoxyribonucleoside 5'-diphosphates (dNTP) required for DNA synthesis and repair that is required for DNA synthesis and repair [PMID:20825972, 19250552]. RRM2 is only expressed during the late G1/early S phase, and degraded in late S phase, and the activity of RNR, and therefore DNA synthesis and cell proliferation, is controlled during the cell cycle by the synthesis and degradation of RRM2 subunit [PMID:3894352].

实验方案

Product Specific Protocols
IF protocol for CL594 RRM2 antibody CL594-67006Download protocol
FC protocol for CL594 RRM2 antibody CL594-67006Download protocol
Standard Protocols
Click here to view our Standard Protocols
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