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CoraLite® Plus 488-conjugated RRM2 Polyclonal antibody

RRM2 Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-11661

Print datasheet

Synonyms

R2, RR2, RR2M, RRM2

验证数据展示

  • IF Staining of HepG2 using CL488-11661

    IF Staining of HepG2 using CL488-11661

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 RRM2 antibody (CL488-11661) at dilution of 1:0.

  • FC experiment of HepG2 using CL488-11661

    FC experiment of HepG2 using CL488-11661

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human RRM2 (CL488-11661) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 11661-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11661 targets RRM2 in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human
Immunogen RRM2 fusion protein Ag2203 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribonucleotide reductase M2 polypeptide
Synonyms R2, RR2, RR2M, RRM2
Calculated Molecular Weight 389 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC030154
Gene Symbol RRM2
Gene ID (NCBI) 6241
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31350
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribonucleotide reductase M2 subunit is one of two subunits that constitute ribonucleotide reductase, the enzyme that catalyzes the conversion of ribonucleotide 5'-diphosphates into 2'-deoxyribonucleotides, a rate-limiting step in the production of 2'-deoxyribonucleoside 5'-diphosphates (dNTP) required for DNA synthesis and repair that is required for DNA synthesis and repair [PMID:20825972, 19250552]. RRM2 is only expressed during the late G1/early S phase, and degraded in late S phase, and the activity of RNR, and therefore DNA synthesis and cell proliferation, is controlled during the cell cycle by the synthesis and degradation of RRM2 subunit [PMID:3894352].

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RRM2 antibody CL488-11661Download protocol
FC protocol for CL Plus 488 RRM2 antibody CL488-11661Download protocol
Standard Protocols
Click here to view our Standard Protocols