验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy RPS4X Ready-To-Use IHC Kit. RPS4X primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 85040-5-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-85040-5 targets RPS4X in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | RPS4X fusion protein Ag6513 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S4, X-linked |
| 别名 | 242697E12, 40S ribosomal protein S4, Small ribosomal subunit protein eS4, X isoform |
| 计算分子量 | 263 aa, 30 kDa |
| 观测分子量 | 28-30 kDa |
| GenBank蛋白编号 | BC000472 |
| 基因名称 | RPS4X |
| Gene ID (NCBI) | 6191 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P62701 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RPS4X, a X-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). (PMID:8358435)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RPS4X antibody CL488-85040-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |