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验证数据展示

  • WB analysis using 85040-5-RR

    WB analysis using 85040-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85040-5-RR (SCAR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 85040-5-RR

    IF Staining of MCF-7 using 85040-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using SCAR antibody (85040-5-RR, Clone: 242697E12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 85040-5-RR

    FC experiment of HeLa using 85040-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPS4X Recombinant antibody (85040-5-RR, Clone:242697E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 85040-5-RR

    Affinity and Kinetic Characterization of 85040-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85040-5-RR against Human RPS4X were performed. The affinity constant is below 1 pM.

RPS4X Recombinant antibody

RPS4X Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 85040-5-RR

产品说明书

CloneNo. 242697E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), ELISA

别名

242697E12, 40S ribosomal protein S4, Small ribosomal subunit protein eS4, X isoform

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-29 cells, HepG2 cells, HeLa cells, MCF-7 cells, mouse spleen tissue, mouse brain tissue, rat liver tissue
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RPS4X Ready-To-Use IHC Kit. RPS4X primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85040-5-RR targets RPS4X in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RPS4X fusion protein Ag6513 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein S4, X-linked
别名 242697E12, 40S ribosomal protein S4, Small ribosomal subunit protein eS4, X isoform
计算分子量 263 aa, 30 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC000472
基因名称 RPS4X
Gene ID (NCBI) 6191
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP62701
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS4X, a X-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). (PMID:8358435)

实验方案

Product Specific Protocols
WB protocol for RPS4X antibody 85040-5-RRDownload protocol
IF protocol for RPS4X antibody 85040-5-RRDownload protocol
FC protocol for RPS4X antibody 85040-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols