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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 85040-5-RR (same clone as 85040-5-PBS)

    WB analysis using 85040-5-RR (same clone as 85040-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85040-5-RR (SCAR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85040-5-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 85040-5-RR (same clone as 85040-5-PBS)

    IF Staining of MCF-7 using 85040-5-RR (same clone as 85040-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using SCAR antibody (85040-5-RR, Clone: 242697E12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85040-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 85040-5-RR (same clone as 85040-5-PBS)

    FC experiment of HeLa using 85040-5-RR (same clone as 85040-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPS4X Recombinant antibody (85040-5-RR, Clone:242697E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85040-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85040-5-RR

    Affinity and Kinetic Characterization of 85040-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85040-5-RR against Human RPS4X were performed. The affinity constant is below 1 pM.

RPS4X Recombinant antibody, PBS Only

RPS4X Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 85040-5-PBS

产品说明书

CloneNo. 242697E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

242697E12, 40S ribosomal protein S4, Small ribosomal subunit protein eS4, X isoform

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RPS4X Ready-To-Use IHC Kit. RPS4X primary antibody included.

产品信息

85040-5-PBS targets RPS4X in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RPS4X fusion protein Ag6513 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein S4, X-linked
别名 242697E12, 40S ribosomal protein S4, Small ribosomal subunit protein eS4, X isoform
计算分子量 263 aa, 30 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC000472
基因名称 RPS4X
Gene ID (NCBI) 6191
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP62701
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RPS4X, a X-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). (PMID:8358435)