验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, Jurkat cells, HepG2 cells, HeLa cells, mouse spleen tissue, rat spleen tissue, fetal human brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85685-1-RR targets RPL10A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | RPL10A fusion protein Ag10009 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ribosomal protein L10a |
| 别名 | 60S ribosomal protein L10a, Csa 19, CSA-19, Large ribosomal subunit protein uL1, NEDD 6 |
| 计算分子量 | 25 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC006791 |
| 基因名称 | RPL10A |
| Gene ID (NCBI) | 4736 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P62906 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RPL10A (Ribosomal Protein L10a) is a component of the 60S subunit of the ribosome and belongs to the L1P family of ribosomal proteins. It is located in the cytoplasm. RPL10A is ubiquitously expressed in various tissues, it plays a crucial role in ribosome biogenesis and protein synthesis. In addition to its role in protein synthesis, RPL10A has been implicated in cell proliferation, embryogenesis, and organogenesis. PMID: 29953919
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPL10A antibody 85685-1-RR | Download protocol |
| IF protocol for RPL10A antibody 85685-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |