验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-85685 targets RPL10A in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag10009 Product name: Recombinant human RPL10A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-217 aa of BC006791 Sequence: MSSKVSRDTLYEAVREVLHGNQRKRRKFLETVELQISLKNYDPQKDKRFSGTVRLKSTPRPKFSVCVLGDQQHCDEAKAVDIPHMDIEALKKLNKNKKLVKKLAKKYDAFLASESLIKQIPRILGPGLNKAGKFPSLLTHNENMVAKVDEVKSTIKFQMKKVLCLAVAVGHVKMTDDELVYNIHLAVNFLVSLLKKNWQNVRALYIKSTMGKPQRLY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ribosomal protein L10a |
| 别名 | 60S ribosomal protein L10a, Csa 19, CSA-19, Large ribosomal subunit protein uL1, NEDD 6 |
| 计算分子量 | 25 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC006791 |
| 基因名称 | RPL10A |
| Gene ID (NCBI) | 4736 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P62906 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RPL10A (Ribosomal Protein L10a) is a component of the 60S subunit of the ribosome and belongs to the L1P family of ribosomal proteins. It is located in the cytoplasm. RPL10A is ubiquitously expressed in various tissues, it plays a crucial role in ribosome biogenesis and protein synthesis. In addition to its role in protein synthesis, RPL10A has been implicated in cell proliferation, embryogenesis, and organogenesis. PMID: 29953919
