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验证数据展示

  • WB analysis using 83650-1-RR (same clone as 83650-1-PBS)

    WB analysis using 83650-1-RR (same clone as 83650-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83650-1-RR (RNASET2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83650-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00620-1

    Sandwich ELISA standard curve of MP00620-1

    Sandwich ELISA standard curve of MP00620-1, Human RNASET2 Recombinant Matched Antibody Pair - PBS only. 83650-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag28353. 83650-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP00620-1

    Sample test of MP00620-1

    Serum of sixteen individual healthy human donors was measured. The RNASET2 concentration of detected samples was determined to be 0.86 ng/mL with a range of 0.10-4.68 ng/mL.

  • Cytometric bead array standard curve of MP00620-1

    Cytometric bead array standard curve of MP00620-1

    Cytometric bead array standard curve of MP00620-1, RNASET2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83650-2-PBS. Detection antibody: 83650-1-PBS. Standard: Ag28353. Range: 3.125-200 ng/mL.

  • Affinity and Kinetic Characterization of 83650-1-RR

    Affinity and Kinetic Characterization of 83650-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83650-1-RR against Human RNASET2 were performed. The affinity constant is 2.09 nM.

RNASET2 Recombinant monoclonal antibody, PBS Only (Detector)

RNASET2 Uni-rAb® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83650-1-PBS

产品说明书

CloneNo. 240581C4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

RNase T2, RNASE6PL, Ribonuclease T2, Ribonuclease 6, EC:4.6.1.19

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RNASET2 Ready-To-Use IHC Kit. RNASET2 primary antibody included.

产品信息

83650-1-PBS targets RNASET2 as part of a matched antibody pair:

MP00620-1: 83650-2-PBS capture and 83650-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原

CatNo: Ag28353

Product name: Recombinant human RNASET2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 25-256 aa of BC039713

Sequence: DKRLRDNHEWKKLIMVQHWPETVCEKIQNDCRDPPDYWTIHGLWPDKSEGCNRSWPFNLEEIKDLLPEMRAYWPDVIHSFPNRSRFWKHEWEKHGTCAAQVDALNSQKKYFGRSLELYRELDLNSVLLKLGIKPSINYYQVADFKDALARVYGVIPKIQCLPPSQDEEVQTIGQIELCLTKQDQQLQNCTEPGEQPSPKQEVWLANGAAESRGLRVCEDGPVFYPPPKKTKH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribonuclease T2
别名 RNase T2, RNASE6PL, Ribonuclease T2, Ribonuclease 6, EC:4.6.1.19
计算分子量 256 aa, 29 kDa
GenBank蛋白编号BC039713
基因名称 RNase T2
Gene ID (NCBI) 8635
RRIDAB_3671259
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO00584
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNASET2(Ribonuclease T2) is also named as RNASE6PL(Ribonuclease 6) and belongs to the RNase T2 family. It is a unique member of the growing family of tumor-antagonizing genes and behaves as a class II tumor suppressor and abolish the tumorigenic potential of an ovarian cancer-derived cell line. This protein has 2 isoforms produced by alternative splicing. Both mildly glycosylated (ranging from38 to 45 kDa) and highly glycosylated forms (ranging from 50 to 80 kDa) of human tumor suppressor protein RNASET2 can be detected in immunoblotting in P. Pastoris(PMID: 21446958).

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