ChromoTek RFP-Booster Alexa Fluor® 568

anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins

Specificity

mCherry, mPlum, mRFP

Applications

IF, IHC, ICC

Conjugate

Alexa Fluor® 568

Host

Alpaca

Cat No : rb2AF568

Synonyms

RFP, RFP-Booster, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag



Product Information

anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins

DescriptionThe RFP-Booster stabilizes, enhances, and reactivates the signal of RFP-fusion proteins such as mCherry, dsRed, mRFP, etc.. Due to its small size, the RFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy:

• Considerably higher tissue penetration rates

• Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

• Less than 2 nm epitope-label displacement minimizes linkage error

• Monovalent VHHs do not cluster their epitopes

• Validation: structure and function characterized

• Consistent and reliable performance due to recombinant production

ApplicationsIF, IHC, ICC
HostAlpaca
Specificity/TargetmCherry, mPlum, mRFP, mRFPruby, mKate2
For the complete list, please click here: Fluorescent protein specificity table Nano-Booster
ConjugateAlexa Fluor® 568
Physical StateLiquid
Suggested DilutionIF/ICC/IHC: 1:500- 1:1,000
Type Nanobody
ClassRecombinant
DOL2 fluorophores per Nanobody
RRIDAB_2827576
Affinity (KD) Dissociation constant KD of 5nM
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA
Preservative: 0.09% Sodium azide
Storage ConditionShipped at ambient temperature. Store at -20°C/-4°F. Stable for 6 month. Protect from light.
NoteThis product is for research use only, not for diagnostic or therapeutic use

Publications

ApplicationTitle
IF

Dev Cell

Mauve/LYST limits fusion of lysosome-related organelles and promotes centrosomal recruitment of microtubule nucleating proteins.

Authors - Ramona Lattao
IF

Elife

A light-gated transcriptional recorder for detecting cell-cell contacts.

Authors - Kelvin F Cho
IF

Cell Rep

Targeting the tamoxifen receptor within sodium channels to block osteoarthritic pain

Authors - Megan M Mccollum
FC

Cell

Sublethal cytochrome c release generates drug-tolerant persister cells

Authors - Halime Kalkavan
IF

Dev Cell

MYO10-filopodia support basement membranes at pre-invasive tumor boundaries

Authors - Emilia Peuhu