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Validation Data Gallery

  • Confocal images of HeLa cells transiently transfected with LC3B-mCherry (red) and immunostained with RFP-Booster Alexa Fluor647 (magenta). Nuclei were stained with DAPI (blue). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Confocal images of HeLa cells transiently transfected with LC3B-mCherry (red) and immunostained with RFP-Booster Alexa Fluor647 (magenta). Nuclei were stained with DAPI (blue). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

ChromoTek RFP-Booster Alexa Fluor® 647

anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins
Cat No. rb2AF647
引用文献(3)

Specificity

mCherry, mPlum, mRFP

Applications

IF, IHC, ICC

Host

Alpaca

Synonyms

RFP, RFP-Booster, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag

Formats:  Alexa Fluor® 647
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Product Information

anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins

DescriptionThe RFP-Booster stabilizes, enhances, and reactivates the signal of RFP-fusion proteins such as mCherry, mRFP, etc.. Due to its small size, the RFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy:

• Considerably higher tissue penetration rates

• Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

• Less than 2 nm epitope-label displacement minimizes linkage error

• Monovalent VHHs do not cluster their epitopes

• Validation: structure and function characterized

• Consistent and reliable performance due to recombinant production

ApplicationsIF, IHC, ICC
HostAlpaca
Specificity/TargetmCherry, mPlum, mRFP, mRFPruby, mKate2
For the complete list, please click here: Fluorescent protein specificity table Nano-Booster
ConjugateAlexa Fluor® 647
Physical StateLiquid
Suggested DilutionIF/ICC/IHC: 1:500- 1:1,000
Type Nanobody
FormatAlpaca single domain antibody, Nanobody or VHH; monovalent
ClassRecombinant
DOL2 fluorophores per Nanobody
RRIDAB_2827577
Affinity (KD)Dissociation constant KD of 5nM
Excitation/ EmissionExcitation max: 650 nm, Emission max: 665 nm
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA
Preservative: 0.09% Sodium azide
Storage ConditionShipped at ambient temperature. Store at -20°C/-4°F. Stable for 6 month. Protect from light.
Size10 μL; 50 μL
NoteThis product is for research use only, not for diagnostic or therapeutic use

Publications

ApplicationTitle
IF

Nature

Reducing affinity as a strategy to boost immunomodulatory antibody agonism

Authors - Xiaojie Yu
View Article

Molecules

Single-Molecule Localization Microscopy to Study Protein Organization in the Filamentous Fungus Trichoderma atroviride.

Authors - Alexander W A F Reismann
View Article
IF

Life Sci

Nicotine induces senescence in spermatogonia stem cells by disrupting homeostasis between circadian oscillation and rhythmic mitochondrial dynamics via the SIRT6/Bmal1 pathway

Authors - Zelin Zhang
View Article