• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HeLa using CL488-85945

    IF Staining of HeLa using CL488-85945

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RDBP antibody (CL488-85945, Clone: 250420G2 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated RDBP Recombinant antibody

RDBP Recombinant Antibody for IF/ICC
Cat No. CL488-85945

产品说明书

CloneNo. 250420G2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

NELFE, NELF E, NELF-E, RD, RNA-binding protein RD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NELFE Ready-To-Use IHC Kit. NELFE primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 85945-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-85945 targets RDBP in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 RDBP fusion protein Ag1186 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RD RNA binding protein
别名 NELFE, NELF E, NELF-E, RD, RNA-binding protein RD
计算分子量 37 kDa, 43 kDa
观测分子量42 kDa
GenBank蛋白编号BC011600
基因名称 NELFE
Gene ID (NCBI) 7936
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP18615
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RDBP antibody CL488-85945Download protocol
Standard Protocols
Click here to view our Standard Protocols