• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85945-1-RR

    WB analysis using 85945-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85945-1-RR (RDBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85945-1-RR

    WB analysis using 85945-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85945-1-RR (RDBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 85945-1-RR

    IF Staining of HeLa using 85945-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RDBP antibody (85945-1-RR, Clone: 250420G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

RDBP Recombinant antibody

RDBP Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85945-1-RR

产品说明书

CloneNo. 250420G2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

NELFE, NELF E, NELF-E, RD, RNA-binding protein RD

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, Calu-3 cells, mouse brain tissue, rat brain tissue, HEK-293 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NELFE Ready-To-Use IHC Kit. NELFE primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2500-1:7500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85945-1-RR targets RDBP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RDBP fusion protein Ag1186 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RD RNA binding protein
别名 NELFE, NELF E, NELF-E, RD, RNA-binding protein RD
计算分子量 37 kDa, 43 kDa
观测分子量42 kDa
GenBank蛋白编号BC011600
基因名称 NELFE
Gene ID (NCBI) 7936
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP18615
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.

实验方案

Product Specific Protocols
WB protocol for RDBP antibody 85945-1-RRDownload protocol
IF protocol for RDBP antibody 85945-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols