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验证数据展示

  • WB analysis using 85945-1-RR (same clone as 85945-1-PBS)

    WB analysis using 85945-1-RR (same clone as 85945-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85945-1-RR (RDBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85945-1-PBS in a different storage buffer formulation.

  • WB analysis using 85945-1-RR (same clone as 85945-1-PBS)

    WB analysis using 85945-1-RR (same clone as 85945-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85945-1-RR (RDBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85945-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 85945-1-RR (same clone as 85945-1-PBS)

    IF Staining of HeLa using 85945-1-RR (same clone as 85945-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RDBP antibody (85945-1-RR, Clone: 250420G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85945-1-PBS in a different storage buffer formulation.

RDBP Recombinant monoclonal antibody, PBS Only

RDBP Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85945-1-PBS

产品说明书

CloneNo. 250420G2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

NELFE, NELF E, NELF-E, RD, RNA-binding protein RD

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NELFE Ready-To-Use IHC Kit. NELFE primary antibody included.

产品信息

85945-1-PBS targets RDBP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1186

Product name: Recombinant human RDBP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-333 aa of BC011600

Sequence: MLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLYESDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSASPPRSRSRDRSHERNRDRDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RD RNA binding protein
别名 NELFE, NELF E, NELF-E, RD, RNA-binding protein RD
计算分子量 37 kDa, 43 kDa
观测分子量42 kDa
GenBank蛋白编号BC011600
基因名称 NELFE
Gene ID (NCBI) 7936
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP18615
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.

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