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验证数据展示

  • WB analysis of pig kidney using 68126-1-Ig (same clone as 68126-1-PBS)

    WB analysis of pig kidney using 68126-1-Ig (same clone as 68126-1-PBS)

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 68126-1-Ig (RBP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68126-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68126-1-Ig (same clone as 68126-1-PBS)

    IF Staining of HepG2 using 68126-1-Ig (same clone as 68126-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RBP5 antibody (68126-1-Ig, Clone: 1G7E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68126-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 68126-1-Ig (same clone as 68126-1-PBS)

    IF Staining of A431 using 68126-1-Ig (same clone as 68126-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using RBP5 antibody (68126-1-Ig, Clone: 1G7E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68126-1-PBS in a different storage buffer formulation.

RBP5 Monoclonal antibody, PBS Only

RBP5 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68126-1-PBS

产品说明书

CloneNo. 1G7E2

宿主/亚型

Mouse / IgG1

种属反应性

Human, pig

应用

WB, IF/ICC, Indirect ELISA

别名

CRBP III, CRBP3, CRBPIII, HRBPiso, RBP5, Retinol binding protein 5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68126-1-PBS targets RBP5 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, pig samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, pig
免疫原

CatNo: Ag24562

Product name: Recombinant human RBP5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-135 aa of BC029355

Sequence: MPPNLTGYYRFVSQKNMEDYLQALNISLAVRKIALLLKPDKEIEHQGNHMTVRTLSTFRNYTVQFDVGVEFEEDLRSVDGRKCQTIVTWEEEHLVCVQKGEVPNRGWRHWLEGEMLYLELTARDAVCEQVFRKVR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 retinol binding protein 5, cellular
别名 CRBP III, CRBP3, CRBPIII, HRBPiso, RBP5, Retinol binding protein 5
计算分子量 135 aa, 16 kDa
观测分子量 16-18 kDa
GenBank蛋白编号BC029355
基因名称 RBP5
Gene ID (NCBI) 83758
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP82980
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBP5 (CRBP III) belongs to the fatty-acid binding protein (FABP) family. Human liver and kidney contain the highest levels of CRBP III mRNA, and CRBP III is as a major human intracellular carrier of retinol (PMID:11274389). RBP5 iron response are reversible by genetic complementation. And increased RBP5 expression occurs by a post-transcriptional feedback mechanism whereby RBP5 interacts with its own, and with PAP2 mRNAs. RBP5 is the most significant of the proteins upregulated by iron starvation (PMID:34161395). RBP5 has an estimated molecular mass (15.9 kDa) typical of this protein superfamily (PMID:11274389).

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