验证数据展示
经过测试的应用
| Positive WB detected in | pig kidney tissue, pig liver tissue, pig liver tisse |
| Positive IF/ICC detected in | HepG2 cells, A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68126-1-Ig targets RBP5 in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | Human, pig |
| 免疫原 |
CatNo: Ag24562 Product name: Recombinant human RBP5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-135 aa of BC029355 Sequence: MPPNLTGYYRFVSQKNMEDYLQALNISLAVRKIALLLKPDKEIEHQGNHMTVRTLSTFRNYTVQFDVGVEFEEDLRSVDGRKCQTIVTWEEEHLVCVQKGEVPNRGWRHWLEGEMLYLELTARDAVCEQVFRKVR 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | retinol binding protein 5, cellular |
| 别名 | CRBP III, CRBP3, CRBPIII, HRBPiso, RBP5, Retinol binding protein 5 |
| 计算分子量 | 135 aa, 16 kDa |
| 观测分子量 | 16-18 kDa |
| GenBank蛋白编号 | BC029355 |
| 基因名称 | RBP5 |
| Gene ID (NCBI) | 83758 |
| RRID | AB_2935257 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P82980 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RBP5 (CRBP III) belongs to the fatty-acid binding protein (FABP) family. Human liver and kidney contain the highest levels of CRBP III mRNA, and CRBP III is as a major human intracellular carrier of retinol (PMID:11274389). RBP5 iron response are reversible by genetic complementation. And increased RBP5 expression occurs by a post-transcriptional feedback mechanism whereby RBP5 interacts with its own, and with PAP2 mRNAs. RBP5 is the most significant of the proteins upregulated by iron starvation (PMID:34161395). RBP5 has an estimated molecular mass (15.9 kDa) typical of this protein superfamily (PMID:11274389).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RBP5 antibody 68126-1-Ig | Download protocol |
| WB protocol for RBP5 antibody 68126-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
