验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells, A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-68126 targets RBP5 in IF/ICC applications and shows reactivity with Human, pig samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | Human, pig |
免疫原 | RBP5 fusion protein Ag24562 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | retinol binding protein 5, cellular |
别名 | CRBP III, CRBP3, CRBPIII, HRBPiso, RBP5, Retinol binding protein 5 |
计算分子量 | 135 aa, 16 kDa |
观测分子量 | 16-18 kDa |
GenBank蛋白编号 | BC029355 |
基因名称 | RBP5 |
Gene ID (NCBI) | 83758 |
RRID | AB_3084408 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P82980 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RBP5 (CRBP III) belongs to the fatty-acid binding protein (FABP) family. Human liver and kidney contain the highest levels of CRBP III mRNA, and CRBP III is as a major human intracellular carrier of retinol (PMID:11274389). RBP5 iron response are reversible by genetic complementation. And increased RBP5 expression occurs by a post-transcriptional feedback mechanism whereby RBP5 interacts with its own, and with PAP2 mRNAs. RBP5 is the most significant of the proteins upregulated by iron starvation (PMID:34161395). RBP5 has an estimated molecular mass (15.9 kDa) typical of this protein superfamily (PMID:11274389).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 RBP5 antibody CL488-68126 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |