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验证数据展示

  • WB analysis of pig kidney using 68126-1-Ig

    WB analysis of pig kidney using 68126-1-Ig

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 68126-1-Ig (RBP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68126-1-Ig

    IF Staining of HepG2 using 68126-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RBP5 antibody (68126-1-Ig, Clone: 1G7E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of A431 using 68126-1-Ig

    IF Staining of A431 using 68126-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using RBP5 antibody (68126-1-Ig, Clone: 1G7E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

RBP5 Monoclonal antibody

RBP5 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68126-1-Ig

产品说明书

CloneNo. 1G7E2

宿主/亚型

Mouse / IgG1

种属反应性

Human, pig

应用

WB, IF/ICC, ELISA

别名

CRBP III, CRBP3, CRBPIII, HRBPiso, RBP5, Retinol binding protein 5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig kidney tissue, pig liver tissue, pig liver tisse
Positive IF/ICC detected inHepG2 cells, A431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68126-1-Ig targets RBP5 in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, pig
免疫原 RBP5 fusion protein Ag24562 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 retinol binding protein 5, cellular
别名 CRBP III, CRBP3, CRBPIII, HRBPiso, RBP5, Retinol binding protein 5
计算分子量 135 aa, 16 kDa
观测分子量 16-18 kDa
GenBank蛋白编号BC029355
基因名称 RBP5
Gene ID (NCBI) 83758
RRIDAB_2935257
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP82980
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBP5 (CRBP III) belongs to the fatty-acid binding protein (FABP) family. Human liver and kidney contain the highest levels of CRBP III mRNA, and CRBP III is as a major human intracellular carrier of retinol (PMID:11274389). RBP5 iron response are reversible by genetic complementation. And increased RBP5 expression occurs by a post-transcriptional feedback mechanism whereby RBP5 interacts with its own, and with PAP2 mRNAs. RBP5 is the most significant of the proteins upregulated by iron starvation (PMID:34161395). RBP5 has an estimated molecular mass (15.9 kDa) typical of this protein superfamily (PMID:11274389).

实验方案

Product Specific Protocols
WB protocol for RBP5 antibody 68126-1-IgDownload protocol
IF protocol for RBP5 antibody 68126-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols