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验证数据展示

  • IF Staining of A431 using CL488-84104-4

    IF Staining of A431 using CL488-84104-4

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 RBM10 antibody (CL488-84104-4, Clone: 241151G11 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated RBM10 Recombinant monoclonal antibody

RBM10 Recombinant Antibody for IF/ICC
Cat No. CL488-84104-4

产品说明书

CloneNo. 241151G11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

KIAA0122, GPATCH9, GPATC9, G patch domain-containing protein 9, DXS8237E

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy RBM10 Ready-To-Use IHC Kit. RBM10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84104-4-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84104-4 targets RBM10 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5788

Product name: Recombinant human RBM10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 601-930 aa of BC024153

Sequence: QSQQYLYWDGERRTYVPALEQSADGHKETGAPSKEGKEKKEKHKTKTAQQIAKDMERWARSLNKQKENFKNSFQPISSLRDDERRESATADAGYAILEKKGALAERQHTSMDLPKLASDDRPSPPRGLVAAYSGESDSEEEQERGGPEREEKLTDWQKLACLLCRRQFPSKEALIRHQQLSGLHKQNLEIHRRAHLSENELEALEKNDMEQMKYRDRAAERREKYGIPEPPEPKRRKYGGISTASVDFEQPTRDGLGSDNIGSRMLQAMGWKEGSGLGRKKQGIVTPIEAQTRVRGSGLGARGSSYGVTSTESYKETLHKTMVTRFNEAQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RNA binding motif protein 10
别名 KIAA0122, GPATCH9, GPATC9, G patch domain-containing protein 9, DXS8237E
计算分子量 104 kDa
观测分子量100-120 kDa
GenBank蛋白编号BC024153
基因名称 RBM10
Gene ID (NCBI) 8241
RRIDAB_3673341
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP98175
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 100-130 kDa (PMID: 25889998 and PMID: 18315527) .

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RBM10 antibody CL488-84104-4Download protocol
Standard Protocols
Click here to view our Standard Protocols
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