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验证数据展示

  • WB analysis using 84104-4-RR (same clone as 84104-4-PBS)

    WB analysis using 84104-4-RR (same clone as 84104-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84104-4-RR (RBM10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84104-4-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 84104-4-RR (same clone as 84104-4-PBS)

    IHC staining of human placenta using 84104-4-RR (same clone as 84104-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84104-4-RR (RBM10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84104-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBM10 antibody included
  • IHC staining of human placenta using 84104-4-RR (same clone as 84104-4-PBS)

    IHC staining of human placenta using 84104-4-RR (same clone as 84104-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84104-4-RR (RBM10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84104-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBM10 antibody included
  • IHC staining of mouse brain using 84104-4-RR (same clone as 84104-4-PBS)

    IHC staining of mouse brain using 84104-4-RR (same clone as 84104-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84104-4-RR (RBM10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84104-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBM10 antibody included
  • IHC staining of mouse brain using 84104-4-RR (same clone as 84104-4-PBS)

    IHC staining of mouse brain using 84104-4-RR (same clone as 84104-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84104-4-RR (RBM10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84104-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBM10 antibody included
  • IF Staining of A431 using 84104-4-RR (same clone as 84104-4-PBS)

    IF Staining of A431 using 84104-4-RR (same clone as 84104-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBM10 antibody (84104-4-RR, Clone: 241151G11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84104-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84104-4-RR

    Affinity and Kinetic Characterization of 84104-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84104-4-RR against Human RBM10 were performed. The affinity constant is 0.33 nM.

RBM10 Recombinant monoclonal antibody, PBS Only

RBM10 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84104-4-PBS

产品说明书

CloneNo. 241151G11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

KIAA0122, GPATCH9, GPATC9, G patch domain-containing protein 9, DXS8237E

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RBM10 Ready-To-Use IHC Kit. RBM10 primary antibody included.

产品信息

84104-4-PBS targets RBM10 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5788

Product name: Recombinant human RBM10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 601-930 aa of BC024153

Sequence: QSQQYLYWDGERRTYVPALEQSADGHKETGAPSKEGKEKKEKHKTKTAQQIAKDMERWARSLNKQKENFKNSFQPISSLRDDERRESATADAGYAILEKKGALAERQHTSMDLPKLASDDRPSPPRGLVAAYSGESDSEEEQERGGPEREEKLTDWQKLACLLCRRQFPSKEALIRHQQLSGLHKQNLEIHRRAHLSENELEALEKNDMEQMKYRDRAAERREKYGIPEPPEPKRRKYGGISTASVDFEQPTRDGLGSDNIGSRMLQAMGWKEGSGLGRKKQGIVTPIEAQTRVRGSGLGARGSSYGVTSTESYKETLHKTMVTRFNEAQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RNA binding motif protein 10
别名 KIAA0122, GPATCH9, GPATC9, G patch domain-containing protein 9, DXS8237E
计算分子量 104 kDa
观测分子量100-120 kDa
GenBank蛋白编号BC024153
基因名称 RBM10
Gene ID (NCBI) 8241
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP98175
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 100-130 kDa (PMID: 25889998 and PMID: 18315527) .

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