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验证数据展示

  • WB analysis using 84104-4-RR

    WB analysis using 84104-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84104-4-RR (RBM10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 84104-4-RR

    IHC staining of human placenta using 84104-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84104-4-RR (RBM10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RBM10 antibody included
  • IHC staining of human placenta using 84104-4-RR

    IHC staining of human placenta using 84104-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84104-4-RR (RBM10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RBM10 antibody included
  • IHC staining of mouse brain using 84104-4-RR

    IHC staining of mouse brain using 84104-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84104-4-RR (RBM10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RBM10 antibody included
  • IHC staining of mouse brain using 84104-4-RR

    IHC staining of mouse brain using 84104-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84104-4-RR (RBM10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RBM10 antibody included
  • IF Staining of A431 using 84104-4-RR

    IF Staining of A431 using 84104-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBM10 antibody (84104-4-RR, Clone: 241151G11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84104-4-RR

    Affinity and Kinetic Characterization of 84104-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84104-4-RR against Human RBM10 were performed. The affinity constant is 0.33 nM.

RBM10 Recombinant monoclonal antibody

RBM10 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84104-4-RR

产品说明书

CloneNo. 241151G11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

KIAA0122, GPATCH9, GPATC9, G patch domain-containing protein 9, DXS8237E

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, MDA-MB-231 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman placenta tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy RBM10 Ready-To-Use IHC Kit. RBM10 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84104-4-RR targets RBM10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5788

Product name: Recombinant human RBM10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 601-930 aa of BC024153

Sequence: QSQQYLYWDGERRTYVPALEQSADGHKETGAPSKEGKEKKEKHKTKTAQQIAKDMERWARSLNKQKENFKNSFQPISSLRDDERRESATADAGYAILEKKGALAERQHTSMDLPKLASDDRPSPPRGLVAAYSGESDSEEEQERGGPEREEKLTDWQKLACLLCRRQFPSKEALIRHQQLSGLHKQNLEIHRRAHLSENELEALEKNDMEQMKYRDRAAERREKYGIPEPPEPKRRKYGGISTASVDFEQPTRDGLGSDNIGSRMLQAMGWKEGSGLGRKKQGIVTPIEAQTRVRGSGLGARGSSYGVTSTESYKETLHKTMVTRFNEAQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RNA binding motif protein 10
别名 KIAA0122, GPATCH9, GPATC9, G patch domain-containing protein 9, DXS8237E
计算分子量 104 kDa
观测分子量100-120 kDa
GenBank蛋白编号BC024153
基因名称 RBM10
Gene ID (NCBI) 8241
RRIDAB_3671671
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP98175
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 100-130 kDa (PMID: 25889998 and PMID: 18315527) .

实验方案

Product Specific Protocols
IF protocol for RBM10 antibody 84104-4-RRDownload protocol
IHC protocol for RBM10 antibody 84104-4-RRDownload protocol
WB protocol for RBM10 antibody 84104-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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