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验证数据展示

  • FC experiment of HEK-293T using CL488-66815

    FC experiment of HEK-293T using CL488-66815

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RARS (CL488-66815, Clone:1C8G4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IF Staining of HeLa using CL488-66815

    IF Staining of HeLa using CL488-66815

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 RARS antibody (CL488-66815, Clone: 1C8G4 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated RARS Monoclonal antibody

RARS Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66815

产品说明书

CloneNo. 1C8G4

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

1C8G4, Arginine--tRNA ligase, cytoplasmic, arginyl tRNA synthetase, Arginyl-tRNA synthetase, ArgRS

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy RARS1 Ready-To-Use IHC Kit. RARS1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66815-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66815 targets RARS in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag26399

Product name: Recombinant human RARS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-190 aa of BC000528

Sequence: MDVLVSECSARLLQQEEEIKSLTAEIDRLKNCGCLGASPNLEQLQEENLKLKYRLNILRKSLQAERNKPTKNMINIISRLQEVFGHAIKAAYPDLENPPLLVTPSQQAKFGDYQCNSAMGISQMLKTKEQKVNPREIAENITKHLPDNECIEKVEIAGPGFINVHLRKDFVSEQLTSLLVNGVQLPALGE

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 arginyl-tRNA synthetase
别名 1C8G4, Arginine--tRNA ligase, cytoplasmic, arginyl tRNA synthetase, Arginyl-tRNA synthetase, ArgRS
计算分子量 75 kDa
观测分子量 67 kDa
GenBank蛋白编号BC000528
基因名称 RARS
Gene ID (NCBI) 5917
RRIDAB_3084275
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP54136
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RARS, also named ArgRS (arginyl-tRNA synthetase), belongs to the class-I aminoacyl-tRNA synthetase family which catalyze the aminoacylation of tRNA by their cognate amino acid. In higher eukaryotes, one of the two arginyl-tRNA synthetases (ArgRSs) has evolved to have an extended N-terminal domain that plays a crucial role in protein synthesis and cell growth and in integration into the multisynthetase complex (MSC) (PMID:25288775). RARS has two isoforms with the molecular weight of 75 and 67 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 RARS antibody CL488-66815Download protocol
IF protocol for CL Plus 488 RARS antibody CL488-66815Download protocol
Standard Protocols
Click here to view our Standard Protocols
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