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  • Featured Product
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RARS Monoclonal antibody

RARS Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C8G4

Cat No : 66815-1-Ig

Print datasheet

Synonyms

Arginine tRNA ligase, arginyl tRNA synthetase, ArgRS, DALRD1, RARS

验证数据展示

  • WB analysis using 66815-1-Ig

    WB analysis using 66815-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66815-1-Ig (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66815-1-Ig

    WB analysis using 66815-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66815-1-Ig (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66815-1-Ig

    WB analysis of HeLa using 66815-1-Ig

    WB result of RARS antibody (66815-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RARS transfected HeLa cells.

  • IF Staining of HeLa using 66815-1-Ig

    IF Staining of HeLa using 66815-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RARS antibody (66815-1-Ig, Clone: 1C8G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, MCF-7 cells, A431 cells, HEK-293 cells, HepG2 cells, NIH/3T3 cells
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RARS1 Ready-To-Use IHC Kit. RARS1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:8000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66815-1-Ig targets RARS in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen RARS fusion protein Ag26399 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name arginyl-tRNA synthetase
Synonyms Arginine tRNA ligase, arginyl tRNA synthetase, ArgRS, DALRD1, RARS
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC000528
Gene Symbol RARS
Gene ID (NCBI) 5917
RRIDAB_2882158
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54136
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RARS, also named ArgRS (arginyl-tRNA synthetase), belongs to the class-I aminoacyl-tRNA synthetase family which catalyze the aminoacylation of tRNA by their cognate amino acid. In higher eukaryotes, one of the two arginyl-tRNA synthetases (ArgRSs) has evolved to have an extended N-terminal domain that plays a crucial role in protein synthesis and cell growth and in integration into the multisynthetase complex (MSC) (PMID:25288775). RARS has two isoforms with the molecular weight of 75 and 67 kDa.

实验方案

Product Specific Protocols
WB protocol for RARS antibody 66815-1-IgDownload protocol
IF protocol for RARS antibody 66815-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols