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验证数据展示

  • WB analysis using 83336-2-RR (same clone as 83336-2-PBS)

    WB analysis using 83336-2-RR (same clone as 83336-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83336-2-RR (RAB13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83336-2-RR (same clone as 83336-2-PBS)

    IF Staining of HeLa using 83336-2-RR (same clone as 83336-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB13 antibody (83336-2-RR, Clone: 240216D10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83336-2-RR (same clone as 83336-2-PBS)

    IF Staining of HeLa using 83336-2-RR (same clone as 83336-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB13 antibody (83336-2-RR, Clone: 240216D10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 83336-2-RR (same clone as 83336-2-PBS)

    FC experiment of K-562 using 83336-2-RR (same clone as 83336-2-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug RAB13 Recombinant antibody (83336-2-RR, Clone:240216D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00354-1

    Cytometric bead array standard curve of MP00354-1

    Cytometric bead array standard curve of MP00354-1, RAB13 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83336-2-PBS. Detection antibody: 83336-1-PBS. Standard: Ag34898. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 83336-2-RR

    Affinity and Kinetic Characterization of 83336-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83336-2-RR against Human RAB13 were performed. The affinity constant is below 1 pM.

RAB13 Recombinant antibody, PBS Only (Capture)

RAB13 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83336-2-PBS

产品说明书

CloneNo. 240216D10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

GIG4, Ras-related protein Rab-13, Ras related protein Rab 13, Cell growth-inhibiting gene 4 protein, Cell growth inhibiting gene 4 protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RAB13 Ready-To-Use IHC Kit. RAB13 primary antibody included.

产品信息

83336-2-PBS targets RAB13 as part of a matched antibody pair:

MP00354-1: 83336-2-PBS capture and 83336-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 RAB13 fusion protein Ag34898 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB13, member RAS oncogene family
别名 GIG4, Ras-related protein Rab-13, Ras related protein Rab 13, Cell growth-inhibiting gene 4 protein, Cell growth inhibiting gene 4 protein
计算分子量 203 aa, 23 kDa
观测分子量23 kDa
GenBank蛋白编号BC000799
基因名称 RAB13
Gene ID (NCBI) 5872
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP51153
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAB13 (Ras-related protein Rab-13) is also named as GIG4. Rab13 is a member of the Rab GTPase family. RAB13 is highly expressed in malignant cells. Rab13 was highly expressed in BCSC of triple-negative breast cancer (TNBC) (PMID: 35395074). RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc (PMID: 37803063). RAB13 enhances its circulation to the plasma membrane to promote breast cancer invasion. By influencing the tumor microenvironment, RAB13 maintains breast cancer stem cells (PMID: 37803063).