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验证数据展示

  • WB analysis using 83336-2-RR

    WB analysis using 83336-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83336-2-RR (RAB13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 83336-2-RR

    WB analysis of HEK-293 using 83336-2-RR

    WB result of RAB13 antibody (83336-2-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB13 transfected HEK-293 cells.

  • IF Staining of HeLa using 83336-2-RR

    IF Staining of HeLa using 83336-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB13 antibody (83336-2-RR, Clone: 240216D10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HeLa using 83336-2-RR

    IF Staining of HeLa using 83336-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB13 antibody (83336-2-RR, Clone: 240216D10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of K-562 using 83336-2-RR

    FC experiment of K-562 using 83336-2-RR

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug RAB13 Recombinant antibody (83336-2-RR, Clone:240216D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83336-2-RR

    Affinity and Kinetic Characterization of 83336-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83336-2-RR against Human RAB13 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

RAB13 Recombinant monoclonal antibody

RAB13 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83336-2-RR

产品说明书

CloneNo. 240216D10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

GIG4, 240216D10, Cell growth inhibiting gene 4 protein, Cell growth-inhibiting gene 4 protein, Ras related protein Rab 13

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, L02 cells, SW480 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy RAB13 Ready-To-Use IHC Kit. RAB13 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83336-2-RR targets RAB13 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34898

Product name: Recombinant human RAB13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 100-200 aa of BC000799

Sequence: QNWMKSIKENASAGVERLLLGNKCDMEAKRKVQKEQADKLAREHGIRFFETSAKSSMNVDEAFSSLARDILLKSGGRRSGNGNKPPSTDLKTCDKKNTNKC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB13, member RAS oncogene family
别名 GIG4, 240216D10, Cell growth inhibiting gene 4 protein, Cell growth-inhibiting gene 4 protein, Ras related protein Rab 13
计算分子量 203 aa, 23 kDa
观测分子量23 kDa
GenBank蛋白编号BC000799
基因名称 RAB13
Gene ID (NCBI) 5872
RRIDAB_3670998
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP51153
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB13 (Ras-related protein Rab-13) is also named as GIG4. Rab13 is a member of the Rab GTPase family. RAB13 is highly expressed in malignant cells. Rab13 was highly expressed in BCSC of triple-negative breast cancer (TNBC) (PMID: 35395074). RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc (PMID: 37803063). RAB13 enhances its circulation to the plasma membrane to promote breast cancer invasion. By influencing the tumor microenvironment, RAB13 maintains breast cancer stem cells (PMID: 37803063).

实验方案

Product Specific Protocols
IF protocol for RAB13 antibody 83336-2-RRDownload protocol
WB protocol for RAB13 antibody 83336-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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