CoraLite®594 Anti-Mouse PLVAP (MECA-32)

PLVAP Monoclonal Antibody for FC
Cat No. CL594-65214

产品说明书

CloneNo. MECA-32

宿主/亚型

Rat / IgG2a, kappa

种属反应性

Mouse

应用

FC

MECA-32, Plvap, PV 1

缓冲液配方:  PBS and Azide
PBS and Azide
规格价格库存


经过测试的应用

Positive FC detected inbEnd.3 cells
For other applications, we recommend the unconjugated version of this antibody, 65214-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 1 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65214 targets PLVAP in FC applications and shows reactivity with Mouse samples.

经测试应用 FC Application Description
经测试反应性 Mouse
免疫原 Mouse lymph node stromal cells 种属同源性预测
宿主/亚型 Rat / IgG2a, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 plasmalemma vesicle associated protein
别名 MECA-32, Plvap, PV 1
GenBank蛋白编号BC013517
基因名称 Plvap
Gene ID (NCBI) 84094
RRIDAB_3084682
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ91VC4
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Plasmalemma vesicle-associated protein (PLVAP), also known as PV-1, PAL-E and MECA-32 antigen, is an endothelial cell-specific type II membrane N-glycosylated glycoprotein with a molecular weight of approximately 50-65 kDa, that forms homodimers in situ (PMID: 7626790; 30231925). The MECA-32 antibody, derived from rats immunized with murine lymph node stroma, shows high specificity for mouse endothelium in both embryonic and mature tissues (PMID: 2683264; 7626790). It recognizes most endothelial cells in the embryonic and in the adult mouse, with the exception of the brain, skeletal, and cardiac muscle, where the MECA-32 antigen has a more restricted distribution. In skeletal and cardiac muscle only small arterioles and venules express the MECA-32 antigen, while in the brain its expression is negatively correlated with the differentiation of the vasculature to form the blood brain barrier (PMID: 7626790).

实验方案

Product Specific Protocols
FC protocol for CL594 PLVAP antibody CL594-65214Download protocol
Standard Protocols
Click here to view our Standard Protocols
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