验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse small intestine tissue |
| Positive IF-Fro detected in | mouse small intestine tissue |
| Positive FC detected in | bEnd.3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:125-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:125-1:500 |
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
65214-1-Ig targets PLVAP in IF-P, IF-Fro, FC applications and shows reactivity with mouse samples.
| 经测试应用 | IF-P, IF-Fro, FC Application Description |
| 经测试反应性 | mouse |
| 免疫原 | Mouse lymph node stromal cells 种属同源性预测 |
| 宿主/亚型 | Rat / IgG2a, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | plasmalemma vesicle associated protein |
| 别名 | MECA32, MECA-32, Panendothelial cell antigen, Plasmalemma vesicle protein 1, PV 1 |
| GenBank蛋白编号 | BC013517 |
| 基因名称 | Plvap |
| Gene ID (NCBI) | 84094 |
| RRID | AB_3084905 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q91VC4 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
Plasmalemma vesicle-associated protein (PLVAP), also known as PV-1, PAL-E and MECA-32 antigen, is an endothelial cell-specific type II membrane N-glycosylated glycoprotein with a molecular weight of approximately 50-65 kDa, that forms homodimers in situ (PMID: 7626790; 30231925). The MECA-32 antibody, derived from rats immunized with murine lymph node stroma, shows high specificity for mouse endothelium in both embryonic and mature tissues (PMID: 2683264; 7626790). It recognizes most endothelial cells in the embryonic and in the adult mouse, with the exception of the brain, skeletal, and cardiac muscle, where the MECA-32 antigen has a more restricted distribution. In skeletal and cardiac muscle only small arterioles and venules express the MECA-32 antigen, while in the brain its expression is negatively correlated with the differentiation of the vasculature to form the blood brain barrier (PMID: 7626790).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PLVAP antibody 65214-1-Ig | Download protocol |
| FC protocol for PLVAP antibody 65214-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |