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CoraLite® Plus 488-conjugated Phospho-STK11/LKB1 (Thr189) Recombinant antibody

Phospho-STK11/LKB1 (Thr189) Recombinant Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

FC (Intra), IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5J9

Cat No : CL488-80127

Print datasheet

Synonyms

LKB1, Phospho-STK11 (Thr189), PJS, serine/threonine kinase 11, STK11

验证数据展示

  • IF Staining of PC-3 using CL488-80127

    IF Staining of PC-3 using CL488-80127

    Immunofluorescent analysis of (4% PFA) fixed non-treated PC-3 cells and Calyculin A treated PC-3 cells using CoraLite® Plus 488 Phospho-STK11/LKB1 (Thr189) antibody (CL488-80127, Clone: 5J9 ) at dilution of 1:100.

  • FC experiment of PC-3 using CL488-80127

    FC experiment of PC-3 using CL488-80127

    1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite® Plus 488 Anti-Human Phospho-STK11/LKB1 (Thr189) (CL488-80127, Clone:5J9), or 0.13 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

经过测试的应用

Positive IF detected inCalyculin A treated PC-3 cells
Positive FC detected inCalyculin A treated PC-3 cells
Planning an IHC experiment? We recommend our IHCeasy STK11 Ready-To-Use IHC Kit. STK11 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80127-1-RR

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

CL488-80127 targets Phospho-STK11/LKB1 (Thr189) in IF, FC (Intra) applications and shows reactivity with Human, Mouse samples.

Tested Applications FC (Intra), IF
Tested Reactivity Human, Mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name serine/threonine kinase 11
Synonyms LKB1, Phospho-STK11 (Thr189), PJS, serine/threonine kinase 11, STK11
Calculated molecular weight 49 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC007981
Gene symbol STK11
UniprotQ15831
Gene ID (NCBI) 6794
RRIDAB_2919574
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Phospho-STK11/LKB1 (Thr189) antibody CL488-80127Download protocol
Standard Protocols
Click here to view our Standard Protocols