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验证数据展示

  • WB analysis using 81251-2-RR (same clone as 81251-2-PBS)

    WB analysis using 81251-2-RR (same clone as 81251-2-PBS)

    Non-treated NIH/3T3 cells, phosphatase inhibitor treated and λ phosphatase NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81251-2-RR (Phospho-PERK/EIF2AK3 (Ser719) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 81251-2-PBS in a different storage buffer formulation.

  • FC experiment of NIH/3T3 using 81251-2-RR (same clone as 81251-2-PBS)

    FC experiment of NIH/3T3 using 81251-2-RR (same clone as 81251-2-PBS)

    1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-PERK/EIF2AK3 (Ser719) Recombinant monoclonal antibody (81251-2-RR, Clone:243165A4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026). This data was developed using the same antibody clone with 81251-2-PBS in a different storage buffer formulation.

Phospho-PERK/EIF2AK3 (Ser719) Recombinant monoclonal antibody, PBS Only

Phospho-PERK/EIF2AK3 (Ser719) Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 81251-2-PBS

产品说明书

CloneNo. 243165A4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Indirect ELISA

别名

P EIF2AK3 (Ser719), P PERK/EIF2AK3 (Ser719), P-EIF2AK3 (Ser719), Phos EIF2AK3 (Ser719), Phos PERK/EIF2AK3 (Ser719)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

81251-2-PBS targets Phospho-PERK/EIF2AK3 (Ser719) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eukaryotic translation initiation factor 2-alpha kinase 3
别名 P EIF2AK3 (Ser719), P PERK/EIF2AK3 (Ser719), P-EIF2AK3 (Ser719), Phos EIF2AK3 (Ser719), Phos PERK/EIF2AK3 (Ser719)
计算分子量 1116 aa, 125 kDa
观测分子量140 kDa
GenBank蛋白编号BC126354
基因名称 PERK
Gene ID (NCBI) 9451
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NZJ5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)

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