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验证数据展示

  • WB analysis using 81251-2-RR

    WB analysis using 81251-2-RR

    Non-treated NIH/3T3 cells, phosphatase inhibitor treated and λ phosphatase NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81251-2-RR (Phospho-PERK/EIF2AK3 (Ser719) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as loading control.

  • FC experiment of NIH/3T3 using 81251-2-RR

    FC experiment of NIH/3T3 using 81251-2-RR

    1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-PERK/EIF2AK3 (Ser719) Recombinant monoclonal antibody (81251-2-RR, Clone:243165A4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-PERK/EIF2AK3 (Ser719) Recombinant monoclonal antibody

Phospho-PERK/EIF2AK3 (Ser719) Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 81251-2-RR

产品说明书

CloneNo. 243165A4
引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

P EIF2AK3 (Ser719), P PERK/EIF2AK3 (Ser719), P-EIF2AK3 (Ser719), Phos EIF2AK3 (Ser719), Phos PERK/EIF2AK3 (Ser719)

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

81251-2-RR targets Phospho-PERK/EIF2AK3 (Ser719) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eukaryotic translation initiation factor 2-alpha kinase 3
别名 P EIF2AK3 (Ser719), P PERK/EIF2AK3 (Ser719), P-EIF2AK3 (Ser719), Phos EIF2AK3 (Ser719), Phos PERK/EIF2AK3 (Ser719)
计算分子量 1116 aa, 125 kDa
观测分子量140 kDa
GenBank蛋白编号BC126354
基因名称 PERK
Gene ID (NCBI) 9451
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NZJ5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)

实验方案

Product Specific Protocols
FC protocol for Phospho-PERK/EIF2AK3 (Ser719) antibody 81251-2-RRDownload protocol
WB protocol for Phospho-PERK/EIF2AK3 (Ser719) antibody 81251-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Stress Chaperones

Inhibition of α-1,3-mannosyltransferase sensitizes head and neck squamous cell carcinomas to cetuximab via endoplasmic reticulum stress.

Authors - Yu Hao
View Article
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