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验证数据展示

  • WB analysis of NIH/3T3 using 85223-3-RR (same clone as 85223-3-PBS)

    WB analysis of NIH/3T3 using 85223-3-RR (same clone as 85223-3-PBS)

    Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85223-3-RR (Phospho-PDPK1 (Ser241) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85223-3-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 85223-3-RR (same clone as 85223-3-PBS)

    IF Staining of NIH/3T3 using 85223-3-RR (same clone as 85223-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated NIH/3T3 cells using Phospho-PDPK1 (Ser241) antibody (85223-3-RR, Clone: 250623A11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85223-3-PBS in a different storage buffer formulation.

  • FC experiment of NIH/3T3 using 85223-3-RR (same clone as 85223-3-PBS)

    FC experiment of NIH/3T3 using 85223-3-RR (same clone as 85223-3-PBS)

    1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-PDPK1 (Ser241) Recombinant monoclonal antibody (85223-3-RR, Clone:250623A11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026). This data was developed using the same antibody clone with 85223-3-PBS in a different storage buffer formulation.

Phospho-PDPK1 (Ser241) Recombinant monoclonal antibody, PBS Only

Phospho-PDPK1 (Ser241) Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 85223-3-PBS

产品说明书

CloneNo. 250623A11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

p PDPK1, p PDPK1 (Ser241), PDPK1 (Ser241), Phospho PDPK1 (Ser241), Phospho-PDPK1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PDPK1 Ready-To-Use IHC Kit. PDPK1 primary antibody included.

产品信息

85223-3-PBS targets Phospho-PDPK1 (Ser241) in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 3-phosphoinositide dependent protein kinase-1
别名 p PDPK1, p PDPK1 (Ser241), PDPK1 (Ser241), Phospho PDPK1 (Ser241), Phospho-PDPK1
计算分子量 556 aa, 63 kDa
观测分子量58-68 kDa
GenBank蛋白编号BC012103
基因名称 PDPK1
Gene ID (NCBI) 5170
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO15530
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

3-Phosphoinositide dependent protein kinase-1 (PDK1 or PDPK1) is a serine-threonine kinase belonging to AGC kinase family. PDK1 plays a critical role in establishing ACD (Asymmetric cell division) in the epithelium (PMID: 27184845). The kinase activity of PDK1 depends on phosphatidyl inositol 3-kinase (PI3K), a key intermediate in signaling pathways including those from growth factor receptors and adhesion molecules. Substrates of PDK1, including AKT and the protein kinase C (PKC) isozymes, regulate a number of essential cell functions (PMID: 20027184). PDPK1 has 5 isoforms produced by alternative splicing of 48~63 kDa and is detected as 60-63 kDa.

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