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验证数据展示

  • WB analysis of NIH/3T3 using 85223-3-RR

    WB analysis of NIH/3T3 using 85223-3-RR

    Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85223-3-RR (Phospho-PDPK1 (Ser241) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

  • IF Staining of NIH/3T3 using 85223-3-RR

    IF Staining of NIH/3T3 using 85223-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated NIH/3T3 cells using Phospho-PDPK1 (Ser241) antibody (85223-3-RR, Clone: 250623A11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of NIH/3T3 using 85223-3-RR

    FC experiment of NIH/3T3 using 85223-3-RR

    1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-PDPK1 (Ser241) Recombinant monoclonal antibody (85223-3-RR, Clone:250623A11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-PDPK1 (Ser241) Recombinant monoclonal antibody

Phospho-PDPK1 (Ser241) Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 85223-3-RR

产品说明书

CloneNo. 250623A11
引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

p PDPK1, p PDPK1 (Ser241), PDPK1 (Ser241), Phospho PDPK1 (Ser241), Phospho-PDPK1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells
Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy PDPK1 Ready-To-Use IHC Kit. PDPK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

85223-3-RR targets Phospho-PDPK1 (Ser241) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 3-phosphoinositide dependent protein kinase-1
别名 p PDPK1, p PDPK1 (Ser241), PDPK1 (Ser241), Phospho PDPK1 (Ser241), Phospho-PDPK1
计算分子量 556 aa, 63 kDa
观测分子量58-68 kDa
GenBank蛋白编号BC012103
基因名称 PDPK1
Gene ID (NCBI) 5170
RRIDAB_3744115
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO15530
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

3-Phosphoinositide dependent protein kinase-1 (PDK1 or PDPK1) is a serine-threonine kinase belonging to AGC kinase family. PDK1 plays a critical role in establishing ACD (Asymmetric cell division) in the epithelium (PMID: 27184845). The kinase activity of PDK1 depends on phosphatidyl inositol 3-kinase (PI3K), a key intermediate in signaling pathways including those from growth factor receptors and adhesion molecules. Substrates of PDK1, including AKT and the protein kinase C (PKC) isozymes, regulate a number of essential cell functions (PMID: 20027184). PDPK1 has 5 isoforms produced by alternative splicing of 48~63 kDa and is detected as 60-63 kDa.

实验方案

Product Specific Protocols
FC protocol for Phospho-PDPK1 (Ser241) antibody 85223-3-RRDownload protocol
IF protocol for Phospho-PDPK1 (Ser241) antibody 85223-3-RRDownload protocol
WB protocol for Phospho-PDPK1 (Ser241) antibody 85223-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Pathol Res Pract

Identification of bioactive components in Wuji Wan and their protective effects against gastric ulcer via regulating PIK3CA-mediated PI3K/AKT pathway.

Authors - Guokun Zhang
View Article
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