验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | Calyculin A treated HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy MAPK8/JNK Ready-To-Use IHC Kit. MAPK8/JNK primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80024-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.2 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-80024 targets Phospho-JNK (Tyr185) in FC (Intra) applications and shows reactivity with human, mouse samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase 8 |
| 别名 | JNK (Tyr185), p JNK, p JNK (Tyr185), p JNK Tyr185, Phospho JNK |
| 观测分子量 | 37-45kDa |
| GenBank蛋白编号 | NM_139049 |
| 基因名称 | JNK |
| Gene ID (NCBI) | 5599 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 激发激光 | Red Laser (633 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P45983 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 Phospho-JNK (Tyr185) antibody CL647-80024 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
