验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, Calyculin A treated HEK-293T cells, UV treated HEK-293T cells, UV treated NIH/3T3 cells, Anisomycin treated NIH/3T3 cells |
| Positive IF/ICC detected in | UV treated HEK-293 cells |
| Positive FC (Intra) detected in | Calyculin A treated HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
80024-1-RR targets Phospho-JNK (Tyr185) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, pig, chicken, zebrafish, bovine, sheep |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase 8 |
| 别名 | JNK (Tyr185), p JNK, p JNK (Tyr185), p JNK Tyr185, Phospho JNK |
| 观测分子量 | 37-46 kDa |
| GenBank蛋白编号 | NM_139049 |
| 基因名称 | JNK |
| Gene ID (NCBI) | 5599 |
| RRID | AB_2882943 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P45983 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. In the phosphorylation of JNK, JNK1 and JNK2/3 have molecular weights of 46 and 54 kDa(PMID: 21378396)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-JNK (Tyr185) antibody 80024-1-RR | Download protocol |
| WB protocol for Phospho-JNK (Tyr185) antibody 80024-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer Targeting BATF2-RGS2 axis reduces T-cell exhaustion and restores anti-tumor immunity | ||
J Hepatol Nucleolin lactylation contributes to intrahepatic cholangiocarcinoma pathogenesis via RNA splicing regulation of MADD | ||
Cell Metab Remote limb ischemic conditioning alleviates steatohepatitis via extracellular vesicle-mediated muscle-liver crosstalk | ||
Mil Med Res D-mannose alleviates intervertebral disc degeneration through glutamine metabolism | ||
Cancer Commun (Lond) Reciprocal tumor-platelet interaction through the EPHB1-EFNB1 axis in the liver metastatic niche promotes metastatic tumor outgrowth in pancreatic ductal adenocarcinoma | ||
J Extracell Vesicles S100A9 Aggravates Cardiac Fibrosis by TLR4/PGC-1α Mediated Macrophage-Myofibroblast Crosstalk. |
